Statistical properties of dark matter mini-haloes at z >= 15

Understanding the formation of the first objects in the universe critically depends on knowing whether the properties of small dark matter structures at high-redshift (z > 15) are different from their more massive lower-redshift counterparts. To clarify this point, we performed a high-resolution N-body simulation of a cosmological volume 1 Mpc/h comoving on a side, reaching the highest mass resolution to date in this regime. We make precision measurements of various physical properties that characterize dark matter haloes (such as the virial ratio, spin parameter, shape, and formation times, etc.) for the high-redshift (z > 15) dark matter mini-haloes we find in our simulation, and compare them to literature results and a moderate-resolution comparison run within a cube of side-length 100 Mpc/h. We find that dark matter haloes at high-redshift have a log-normal distribution of the dimensionless spin parameter centered around {\lambda} $\sim$ 0.03, similar to their more massive counterparts. They tend to have a small ratio of the length of the shortest axis to the longest axis (sphericity), and are highly prolate. In fact, haloes of given mass that formed recently are the least spherical, have the highest virial ratios, and have the highest spins. Interestingly, the formation times of our mini-halos depend only very weakly on mass, in contrast to more massive objects. This is expected from the slope of the linear power spectrum of density perturbations at this scale, but despite this difference, dark matter structures at high-redshift share many properties with their much more massive counterparts observed at later times.Comment: 17 pages. Accepted for publication in MNRA

Statistical properties of dark matter mini-haloes and the criterion for HD formation in the early universe

The aim of this work is to explore how dark matter structures and astronomical objects (the first generation of stars) formed in the high-redshift universe. We investigate properties of dark matter mini-haloes and clarify the process of primordial star formation that takes place in different dark matter mini-haloes. The specific questions that we aim to answer in this work include how dark matter mini-haloes found at z >= 15 differ from their more massive lower-redshift counterparts and what determines the amount of HD that forms in primordial gas at the initial stage of protostellar collapse. We ran a high-resolution N-body simulation that has the highest mass resolution ever achieved for a representative cosmological volume at these high redshifts, and made precision measurements of various physical properties that characterise dark matter haloes. As expected from the differences in the slope of the dark matter density power spectrum, the dependence of formation time on dark matter halo mass is very weak in the case of the haloes that we study here. Despite this difference, dark matter structures at high redshift share many properties with their much more massive counterparts that form at later times. We ran a separate set of cosmological hydrodynamical simulations to study gas starting to collapse in dark matter haloes. We found that in some of our simulated mini-haloes, HD cooling became important during the initial collapse, and investigated in detail why this occurred. We compared HD-rich and HD-poor mini-haloes in our simulations and found that the amount of HD that forms is linked to the speed of the gravitational collapse. If the collapse is rapid, dynamical heating prevents the gas from cooling to temperatures low enough for HD cooling to become important, but if the collapse is slow, HD cooling can come to dominate, resulting in a minimum gas temperature which is lower by a factor of two. We investigated what properties of the mini-haloes were responsible for determining the collapse time, and showed that, contrary to previous suggestions, the mass of the mini-halo and the rotational energy of the gas appear to have little in uence on the speed of the collapse. We therefore suspect that the main factor determining whether the collapse is slow or rapid, and hence whether HD cooling becomes important or not, is the degree of turbulence in the gas

Exact anisotropic brane cosmologies

We present exact solutions of the gravitational field equations in the generalized Randall-Sundrum model for an anisotropic brane with Bianchi type I and V geometry, with perfect fluid and scalar fields as matter sources. Under the assumption of a conformally flat bulk (with vanishing Weyl tensor) for a cosmological fluid obeying a linear barotropic equation of state the general solution of the field equations can be expressed in an exact parametric form for both Bianchi type I and V space-times. In the limiting case of a stiff cosmological fluid with pressure equal to the energy density, for a Bianchi type I Universe the solution of the field equations are obtained in an exact analytic form. Several classes of scalar field models evolution on the brane are also considered, corresponding to different choices of the scalar field potential. For all models the behavior of the observationally important parameters like shear, anisotropy and deceleration parameter is considered in detail.Comment: revised version to appear in PR

Stable DHLA–PEG capped PbS quantum dots: from synthesis to near-infrared biomedical imaging

The short shelf-life of water-soluble quantum dots (QDs) due to colloidal instability represents a major drawback to their exploitation. This work examines the colloidal stability of PbS nanoparticles capped with dihydrolipoic acid–polyethylene glycol (DHLA–PEG) ligands terminated with functional groups such as –NH 2 , –COOH, OMe and –N3 and their application for in vivo imaging. We prove a mechanism of colloidal instability and develop a strategy to produce for the first time stable PEG-capped PbS quantum dots with high quantum yield and optical emission in the first and the second near-infrared (NIR) windows of low absorption of biological tissues. The NIR imaging of in vivo biodistribution is demonstrated at wavelengths 4 1000 nm, with benefits of reduced tissue absorption and light scattering. The stability, biocompatibility and potential for further QD functionalization open up realistic prospects for non-invasive bioimaging applications

Right Unitarity Triangles, Stable CP-violating Phases and Approximate Quark-Lepton Complementarity

Current experimental data indicate that two unitarity triangles of the CKM quark flavor mixing matrix V are almost the right triangles with \alpha \approx 90^\circ. We find that \alpha = 90^\circ actually points to {\rm Re}(V_{tb}V_{ud}V^*_{td}V^*_{ub}) = 0. Considering a very suggestive parametrization of V, we show that its CP-violating phase \phi is nearly equal to \alpha (i.e., \phi - \alpha \approx 1.1^\circ). Both \phi and \alpha are stable against the renormalizaton-group evolution from the electroweak scale M_Z to a superhigh energy scale M_X or vice versa, and thus it is impossible to obtain \alpha = 90^\circ at M_Z from \phi = 90^\circ at M_X. We conjecture that there might also exist a maximal CP-violating phase \phi \approx 90^\circ in the MNS lepton flavor mixing matrix U. The approximate quark-lepton complementarity relations, which hold in the standard parametrizations of V and U, can also hold in our particular parametrizations of V and U simply due to the smallness of |V_{ub}| and |V_{e3}|.Comment: RevTex 12 pages, 1 PS figure. Accepted for publication in PL

Cdc42 regulates cell polarization and contractile actomyosin rings during terminal differentiation of human erythroblasts

The molecular mechanisms involved in the terminal differentiation of erythroblasts have been elucidated by comparing enucleation and cell division. Although various similarities and differences between erythroblast enucleation and cytokinesis have been reported, the mechanisms that control enucleation remain unclear. We previously reported that dynein and microtubule-organizing centers mediated the polarization of nuclei in human erythroblasts. Moreover, the accumulation of F-actin was noted during the enucleation of erythroblasts. Therefore, during enucleation, upstream effectors in the signal transduction pathway regulating dynein or actin, such as cell division control protein 42 homolog (Cdc42), may be crucial. We herein investigated the effects of the Cdc42 inhibitor, CASIN, on cytokinesis and enucleation in colony-forming units-erythroid (CFU-Es) and mature erythroblasts (day 10). CASIN blocked the proliferation of CFU-Es and their enucleation in a dose-dependent manner. Dynein adopted an island-like distribution in the cytoplasm of non-treated CFU-Es, but was concentrated near the nucleus as a dot and co-localized with gamma -tubulin in CASIN-treated cells. CASIN blocked the accumulation of F-actin in CFU-Es and day 10 cells. These results demonstrated that Cdc42 plays an important role in cytokinesis, nuclear polarization and nuclear extrusion through a relationship with dynein and actin filament organization during the terminal differentiation of erythroblasts

Cooperation of Mtmr8 with PI3K Regulates Actin Filament Modeling and Muscle Development in Zebrafish

It has been shown that mutations in at least four myotubularin family genes (MTM1, MTMR1, 2 and 13) are causative for human neuromuscular disorders. However, the pathway and regulative mechanism remain unknown.Here, we reported a new role for Mtmr8 in neuromuscular development of zebrafish. Firstly, we cloned and characterized zebrafish Mtmr8, and revealed the expression pattern predominantly in the eye field and somites during early somitogenesis. Using morpholino knockdown, then, we observed that loss-of-function of Mtmr8 led to defects in somitogenesis. Subsequently, the possible underlying mechanism and signal pathway were examined. We first checked the Akt phosphorylation, and observed an increase of Akt phosphorylation in the morphant embryos. Furthermore, we studied the PH/G domain function within Mtmr8. Although the PH/G domain deletion by itself did not result in embryonic defect, addition of PI3K inhibitor LY294002 did give a defective phenotype in the PH/G deletion morphants, indicating that the PH/G domain was essential for Mtmr8's function. Moreover, we investigated the cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development, and found that both Mtmr8-MO1 and Mtmr8-MO2+LY294002 led to the disorganization of the actin cytoskeleton. In addition, we revealed a possible participation of Mtmr8 in the Hedgehog pathway, and cell transplantation experiments showed that Mtmr8 worked in a non-cell autonomous manner in actin modeling.The above data indicate that a conserved functional cooperation of Mtmr8 with PI3K regulates actin filament modeling and muscle development in zebrafish, and reveal a possible participation of Mtmr8 in the Hedgehog pathway. Therefore, this work provides a new clue to study the physiological function of MTM family members

Refraction and reflection of infragravity waves near submarine canyons

Author Posting. © American Geophysical Union, 2007. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research 112 (2007): C10009, doi:10.1029/2007JC004227.The propagation of infragravity waves (ocean surface waves with periods from 20 to 200 s) over complex inner shelf (water depths from about 3 to 50 m) bathymetry is investigated with field observations from the southern California coast. A wave-ray-path-based model is used to describe radiation from adjacent beaches, refraction over slopes (smooth changes in bathymetry), and partial reflection from submarine canyons (sharp changes in bathymetry). In both the field observations and the model simulations the importance of the canyons depends on the directional spectrum of the infragravity wave field radiating from the shoreline and on the distance from the canyons. Averaged over the wide range of conditions observed, a refraction-only model has reduced skill near the abrupt bathymetry, whereas a combined refraction and reflection model accurately describes the distribution of infragravity wave energy on the inner shelf, including the localized effects of steep-walled submarine canyons.Funding was provided by the Office of Naval Research and the National Science Foundation

Ndel1 Promotes Axon Regeneration via Intermediate Filaments

Failure of axons to regenerate following acute or chronic neuronal injury is attributed to both the inhibitory glial environment and deficient intrinsic ability to re-grow. However, the underlying mechanisms of the latter remain unclear. In this study, we have investigated the role of the mammalian homologue of aspergillus nidulans NudE, Ndel1, emergently viewed as an integrator of the cytoskeleton, in axon regeneration. Ndel1 was synthesized de novo and upregulated in crushed and transected sciatic nerve axons, and, upon injury, was strongly associated with neuronal form of the intermediate filament (IF) Vimentin while dissociating from the mature neuronal IF (Neurofilament) light chain NF-L. Consistent with a role for Ndel1 in the conditioning lesion-induced neurite outgrowth of Dorsal Root Ganglion (DRG) neurons, the long lasting in vivo formation of the neuronal Ndel1/Vimentin complex was associated with robust axon regeneration. Furthermore, local silencing of Ndel1 in transected axons by siRNA severely reduced the extent of regeneration in vivo. Thus, Ndel1 promotes axonal regeneration; activating this endogenous repair mechanism may enhance neuroregeneration during acute and chronic axonal degeneration

Restricted Morphological and Behavioral Abnormalities following Ablation of β-Actin in the Brain

The local translation of β-actin is one mechanism proposed to regulate spatially-restricted actin polymerization crucial for nearly all aspects of neuronal development and function. However, the physiological significance of localized β-actin translation in neurons has not yet been demonstrated in vivo. To investigate the role of β-actin in the mammalian central nervous system (CNS), we characterized brain structure and function in a CNS-specific β-actin knock-out mouse (CNS-ActbKO). β-actin was rapidly ablated in the embryonic mouse brain, but total actin levels were maintained through upregulation of other actin isoforms during development. CNS-ActbKO mice exhibited partial perinatal lethality while survivors presented with surprisingly restricted histological abnormalities localized to the hippocampus and cerebellum. These tissue morphology defects correlated with profound hyperactivity as well as cognitive and maternal behavior impairments. Finally, we also identified localized defects in axonal crossing of the corpus callosum in CNS-ActbKO mice. These restricted defects occurred despite the fact that primary neurons lacking β-actin in culture were morphologically normal. Altogether, we identified novel roles for β-actin in promoting complex CNS tissue architecture while also demonstrating that distinct functions for the ubiquitously expressed β-actin are surprisingly restricted in vivo